TRPC5 anticorps (AA 827-845) (FITC)

N° du produit ABIN2485379

Cet anticorps anti-TRPC5 Monoclonal Souris conjugué à FITC (Clone N67-15 (Formerly S67-15)) (ABIN2485379) détecte spécifiquement TRPC5 dans WB, IHC, IP et AA. L’anticorps est réactif avec des échantillons de Humain. Validé indépendamment pour une utilisation en Immunofluorescence.

Aperçu rapide pour TRPC5 anticorps (AA 827-845) (FITC) (ABIN2485379)

Antigène: TRPC5 (Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TRPC5 est conjugé à/à la FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Antibody Array (AA)

Clone: N67-15 (Formerly S67-15)

Détail du produit anti-TRPC5 anticorps (FITC)

Épitope: AA 827-845

Specificité: Detects ~110 kDa.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Synthetic peptide amino acids 827-845 of human TrpC5 (also known as short transient receptor potential channel 5, and Htrp5)

Isotype: IgG2b

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2485379 was sufficient for detection of TrpC5 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Independent Validation (IF)

Validation #103875 (Immunofluorescence)

by: Experimentelle Anästhesiologie, Universitätsklinikum Erlangen

No.: #103875

Date: 10.04.2019

Antigène: TRPC5

Numéro du lot: 1007

Application validée: Immunofluorescence

Contrôle positif:

C57Bl6/J Mouse WT Trigeminal Ganglion (TG)

Contrôle négative:

Mouse TRPC5 knock-out Trigeminal Ganglion

Conclusion:

ABIN2485379 specifically stains TRPC5 positive neurons in fixed murine trigeminal ganglia.

Validation Images

A. Representative image of ophthalmic region of trigeminal ganglion of C57Bl6/J mouse stained with ABIN2485379 diluted 1:500. B. Representative image of Z-Stack of ophthalmic region of trigeminal ganglion of TRPC5 ko stained with ABIN2485379 diluted 1:500.

Protocole

Anticorps primaire: ABIN2485379

Full Protocol:

• Surgical plane of anesthesia with Ketamine and Xylazine.
• Perfuse mouse transcardially with
• • 1ml/g BW ice cold PBS
  • 1ml/g BW 4% PFA.
• Postfix skull base in 4% PFA ON at 4°C.
• Harvest TGs in PBS.
• Wash TGs 4x with PBS for 30min at RT.
• Incubate TGs with rotator in blocking solution (PBS containing 1% BSA, 0.5% Triton X-100) ON at 4°C.
• Incubate TGs with rotator with primary FITC-conjugated mouse anti-TRPC5 antibody (antibodies-online, ABIN2485379, lot 1007) diluted 1:500 in blocking solution ON at 4°C.
• Wash TGs 4x with PBS for 30min at RT.
• Mount TGs with 1% low melting agarose.
• Whole Organ 2-Photon Imaging with a Zeiss LSM880 NLO Intravital Microscope; Settings: Excitation Wavelenth: 940 nm, Detection: 500-550 nm for wt and TRPC5 ko TGs.

Notes:

• In wt mice antibody concentrations of 1:100, 1:500, 1:1000 and 1:2000 were tested with best results using a concentration of 1:500.

• ABIN2485379 is suitable for whole organ IHC. TRPC5 positive Neurons show clear cytoplasmic and membranous staining. No neuronal staining visible. Residues of antibody visible in vasculature due to tissue manipulation during washing.

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur TRPC5

Antigène: TRPC5 (Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5))

Autre désignation: TRPC5

Sujet: Transient receptor potential cation channel, subfamily C, member 5, also known as TRPC5, is a subtype of the TRPC family of mammalian transient receptor potential ion channels. Homo-multimeric TRPC5 and hetero-multimeric TRPC5-TRPC1 channels are activated by extracellular reduced thioredoxin (1). This activation probably plays a role in rheumatoid arthritis. It has also been recently found to be involved in the action on anaesthetics such as chloroform, halothane and propofol (2).

ID gène: 7224

NCBI Accession: NP_036603

UniProt: Q9UL62