Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) anticorps (FITC)

Détails pour le produit réf. ABIN2485379, Fournisseur: Connectez-vous pour afficher
Antigène
  • trpc5
  • wu:fi73f08
  • TRPC5
  • Trpc5
  • TRP5
  • CCE2
  • TRP-5
  • Trrp5
  • trp
  • transient receptor potential cation channel, subfamily C, member 5a
  • transient receptor potential cation channel subfamily C member 5
  • transient receptor potential cation channel, subfamily C, member 5
  • trpc5a
  • TRPC5
  • Trpc5
Épitope
AA 827-845
58
11
9
6
3
3
2
2
2
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
107
63
60
3
2
1
Hôte
Souris
70
44
3
Clonalité (Clone)
Monoclonal ()
Conjugué
FITC
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
1
Application
Antibody Array (AA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
114
87
57
25
24
22
21
17
2
2
1
1
1
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
'Independent Validation' signe
Antigène TRPC5
Numéro du lot 1007
Application validée Immunofluorescence
Contrôle positif

C57Bl6/J Mouse WT Trigeminal Ganglion (TG)

Contrôle négative

Mouse TRPC5 knock-out Trigeminal Ganglion

Conclusion

ABIN2485379 specifically stains TRPC5 positive neurons in fixed murine trigeminal ganglia.

Anticorps primaire ABIN2485379
Protocole
  • Surgical plane of anesthesia with Ketamine and Xylazine.
  • Perfuse mouse transcardially with
    • 1ml/g BW ice cold PBS
    • 1ml/g BW 4% PFA.
  • Postfix skull base in 4% PFA ON at 4°C.
  • Harvest TGs in PBS.
  • Wash TGs 4x with PBS for 30min at RT.
  • Incubate TGs with rotator in blocking solution (PBS containing 1% BSA, 0.5% Triton X-100) ON at 4°C.
  • Incubate TGs with rotator with primary FITC-conjugated mouse anti-TRPC5 antibody (antibodies-online, ABIN2485379, lot 1007) diluted 1:500 in blocking solution ON at 4°C.
  • Wash TGs 4x with PBS for 30min at RT.
  • Mount TGs with 1% low melting agarose.
  • Whole Organ 2-Photon Imaging with a Zeiss LSM880 NLO Intravital Microscope; Settings: Excitation Wavelenth: 940 nm, Detection: 500-550 nm for wt and TRPC5 ko TGs.
Notes
  • In wt mice antibody concentrations of 1:100, 1:500, 1:1000 and 1:2000 were tested with best results using a concentration of 1:500.

  • ABIN2485379 is suitable for whole organ IHC. TRPC5 positive Neurons show clear cytoplasmic and membranous staining. No neuronal staining visible. Residues of antibody visible in vasculature due to tissue manipulation during washing.

Validation Images
Immunofluorescence validation image for anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (FITC) (ABIN2485379) A. Representative image of ophthalmic region of trigeminal ganglion of C57Bl6/J mouse...
Immunogène Synthetic peptide amino acids 827-845 of human TrpC5 (also known as short transient receptor potential channel 5, and Htrp5)
Clone S67-15
Isotype IgG2b
Specificité Detects ~110 kDa.
Purification Protein G Purified
Antigène
Autre désignation TRPC5 (TRPC5 Antibody Extrait)
Sujet Transient receptor potential cation channel, subfamily C, member 5, also known as TRPC5, is a subtype of the TRPC family of mammalian transient receptor potential ion channels. Homo-multimeric TRPC5 and hetero-multimeric TRPC5-TRPC1 channels are activated by extracellular reduced thioredoxin (1). This activation probably plays a role in rheumatoid arthritis. It has also been recently found to be involved in the action on anaesthetics such as chloroform, halothane and propofol (2).
ID gène 7224
NCBI Accession NP_036603
UniProt Q9UL62
Indications d'application
  • WB (1:1000)
  • IHC (1:1000)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Commentaires

1 μg/ml of SMC-344 was sufficient for detection of TrpC5 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (FITC) (ABIN2485379) Immunohistochemistry analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone S67-1...
Western Blotting (WB) image for anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (FITC) (ABIN2485379) Western Blot analysis of Rat Brain Membrane showing detection of ~110 kDa TrpC5 prote...
Avez-vous cherché autre chose?