CLCN3 anticorps (AA 98-115) (Atto 488)

N° du produit ABIN2485471

L’anticorps Souris Monoclonal anti-CLCN3 a été validé pour WB, IHC, ICC et IF. Il convient pour détecter CLCN3 dans des échantillons de Rat.

Aperçu rapide pour CLCN3 anticorps (AA 98-115) (Atto 488) (ABIN2485471)

Antigène: CLCN3 (Chloride Channel 3 (CLCN3))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CLCN3 est conjugé à/à la Atto 488

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S258-5

Détail du produit anti-CLCN3 anticorps (Atto 488)

Épitope: AA 98-115

Specificité: Detects ~90 kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).

Réactivité croisée: Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2485471 was sufficient for detection of Clcn3 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur CLCN3

Antigène: CLCN3 (Chloride Channel 3 (CLCN3))

Autre désignation: ClCN3

Sujet: Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory.

ID gène: 84360

NCBI Accession: NP_445815

UniProt: P51792