Versican anticorps (AA 362-585) (FITC)

N° du produit ABIN2485746

Cet anticorps Souris Monoclonal détecte spécifiquement Versican dans WB, ICC et IF. Il présente une réactivité envers Souris.

Aperçu rapide pour Versican anticorps (AA 362-585) (FITC) (ABIN2485746)

Antigène: Versican (Vcan)

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Versican est conjugé à/à la FITC

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S351-23

Détail du produit anti-Versican anticorps (FITC)

Épitope: AA 362-585

Specificité: Detects >350 kDa.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2485746 was sufficient for detection of Versican in 20 μg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur Versican

Antigène: Versican (Vcan)

Autre désignation: Versican

Sujet: Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a Lectinlike sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length anddegree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in lesions of athero-sclerosis suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may faciliatate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels.

ID gène: 13003

UniProt: Q62059

Pathways: Glycosaminoglycan Metabolic Process