TCP1 alpha/CCTA anticorps (C-Term) (PE)

N° du produit ABIN2485925

Cet anticorps anti-TCP1 alpha/CCTA est un anticorps Rat Monoclonal détectant TCP1 alpha/CCTA dans WB, IF, FACS, IP et ICC. Adapté pour Souris.

Aperçu rapide pour TCP1 alpha/CCTA anticorps (C-Term) (PE) (ABIN2485925)

Antigène: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp TCP1 alpha/CCTA est conjugé à/à la PE

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 91a

Détail du produit anti-TCP1 alpha/CCTA anticorps (PE)

Épitope: C-Term

Specificité: Detects ~60 & ~92 kDa. Cross reactivity with human HSP60 in WB. Reacts weakly with S. cerevisiae, consistent with the epitope being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recog TCP1 of P. sativum, and the sequence of A. thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.

Réactivité croisée: C. elegans, Boeuf (Vache), Chien, Drosophila melanogaster, Cobaye, Hamster, Humain, Singe, Souris, Porc, Lapin, Rat, Saccharomyces cerevisiae

Purification: Protein G Purified

Immunogène: Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).

Isotype: IgG2a

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2485925 was sufficient for detection of TCP1 alpha in 20 μg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur TCP1 alpha/CCTA

Antigène: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Autre désignation: TCP1 alpha

Sujet: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40 % identity to TF55, but only minimal similarity to HSP60 and GroEL.

ID gène: 21454

NCBI Accession: NP_038714

UniProt: P11983