COL3A1 anticorps (Chain alpha 1)

N° du produit ABIN7581223

Cet anticorps Lapin Polyclonal détecte spécifiquement COL3A1 dans WB, IF, IHC (p), IHC (fro), EIA et RIA. Il présente une réactivité avec des échantillons de Souris et Rat.

Aperçu rapide pour COL3A1 anticorps (Chain alpha 1) (ABIN7581223)

Antigène: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp COL3A1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Détail du produit anti-COL3A1 anticorps

Épitope: Chain alpha 1

Specificité: Rat Collagen type III 100 % . Rat Collagen I < 0.1 % Rat Collagen type V < 10 % Rat Elastin < 0.1 % (determined by solid phase RIA at 1/50 dilution).

Purification: Affinity Chromatography

Immunogène: Purified Collagen type III from Rat skin.

Information d'application

Indications d'application: RIA. Western blot. ELISA: >/= 1/200. Immunflourescence: 1/80. Immunohistochemistry on Frozen and Paraffin Embedded Tissues: >/= 1/500. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Incubation Time: 60 minutes at RT or 2-8 °C over night. Positive Control: Rat skin or liver.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water. For further dilutions use appropriate antibody diluent.

Concentration: 1.0 mg/mL

Buffer: PBS without BSA or preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur COL3A1

Antigène: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Sujet: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.Synonyms: COL3A1

ID gène: 12825

NCBI Accession: NP_034060

UniProt: P08121

Pathways: Autophagy, Growth Factor Binding