Xylose anticorps

N° du produit ABIN249329

L’anticorps anti-Xylose Polyclonal Lapin est utilisé pour la détection de Xylose dans des échantillons de Chlamydomonas et Plantes supérieures. Il a été validé pour WB.

Aperçu rapide pour Xylose anticorps (ABIN249329)

Antigène: Xylose

Reactivité: Chlamydomonas, Plantes supérieures

Hôte: Lapin

Clonalité: Polyclonal

Application: Western Blotting (WB)

Détail du produit

Fonction: Anti-Xylose

Specificité: Antibody binds to heavy chains of Goat IgG and light chains of all Goat immunoglobuli

Réactivité croisée (Details): Higher plants and algae
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Higher plants

Purification: Immunogen affinity purified serum

Immunogène: xylose residues bound to the N-glycan in beta 1

Information d'application

Indications d'application: 1 μg/mL (ELISA), 2 μg/10 mL incubation buffer (WB)

Commentaires:

negative control: Fetuin, a glycoprotein containing fucose linked in alpha 1.6 and no xylose, Sigma, product number F3385.positive control: Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Détail du antigène

Antigène: Xylose

Sujet:

Background: This antibody specifically cross-reacts against xylose residues bound to the protein N-glycans in beta1,2. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.

Additional information: Beta (1,2) xylose is present exclusively in plant N-glycans so antibodies against this sugar moiety should not cross-react with any mammal glycoprotein.This antibody do not bind free D-xylose. This antibody does not seem to work in immunolocalization.

Poids moléculaire: 10-100 for various glycoproteins