Fucose anticorps

N° du produit ABIN249420

Cité dans 1 publication. Cet anticorps anti-Fucose Polyclonal Lapin (ABIN249420) détecte spécifiquement Fucose dans ELISA, IL et WB. L’anticorps est réactif avec des échantillons de Diatomées et Plantes supérieures.

Aperçu rapide pour Fucose anticorps (ABIN249420)

Antigène: Fucose

Reactivité: Diatomées, Plantes supérieures

Hôte: Lapin

Clonalité: Polyclonal

Application: ELISA, Immunolocalization (IL), Western Blotting (WB)

Détail du produit

Fonction: Anti-Fucose

Réactivité croisée (Details): Higher plants, Pheodactylum tricornutum (diatom)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Higher plants,Phaeodactylum tricornutum

Purification: Immunogen affinity purified serum

Immunogène: Core fucose residues bound to the N-glycan in alpha 1

Information d'application

Indications d'application: 0.5 μg/mL (ELISA), 1 : 40 (IL), 1 μg/10 mL (WB)

Commentaires:

controls:PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250The antibody does not recognize alpha 1,6-fucose.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Référence

Baïet, Burel, Saint-Jean, Louvet, Menu-Bouaouiche, Kiefer-Meyer, Mathieu-Rivet, Lefebvre, Castel, Carlier, Cadoret, Lerouge, Bardor: "N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme." dans: The Journal of biological chemistry, Vol. 286, Issue 8, pp. 6152-64, (2011) (PubMed).

Détail du antigène

Antigène: Fucose

Sujet:

Background: This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. This residue is characteristic of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.

Additional information: Alpha (1,3) fucose is present not only in plants but also in some invertebrates (such as nematodes, bees, etc,) , However, cross-reaction with glycoproteins from these organisms is weaker than the one observed in plants, This sugar residue does not exist in mammals, in their endogenous glycoproteins

Poids moléculaire: 10 - 100 for various glycoproteins