Fucose anticorps

N° du produit ABIN249420

Détail du produit

Antigène: Fucose

Reactivité: Plantes supérieures

Hôte: Lapin

Clonalité: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Réactivité croisée (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: higher plants

Purification: serum

Immunogène: core fucose residues bound to the N-glycan in alpha 1,3

Information d'application

Indications d'application: 1 μg/10 mL of incubation buffer, with standard ECL (WB), 1: 40 (IL)

Commentaires:

controls:PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250The antibody does not recognize alpha 1,6-fucose.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Buffer: PBS pH 7.4

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Référence

Baïet, Burel, Saint-Jean, Louvet, Menu-Bouaouiche, Kiefer-Meyer, Mathieu-Rivet, Lefebvre, Castel, Carlier, Cadoret, Lerouge, Bardor: "N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme." dans: The Journal of biological chemistry, Vol. 286, Issue 8, pp. 6152-64, (2011) (PubMed).

Détail du antigène

Antigène: Fucose

Sujet: This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.

Poids moléculaire: 10 - 100 for various glycoproteins