GLN1 anticorps (all Isoforms)

N° du produit ABIN2559393

L’anticorps Lapin Polyclonal anti-GLN1 a été validé pour WB. Il convient pour détecter GLN1 dans des échantillons de Arabidopsis thaliana, Spinach et Zea mays. Il y a 1 publication disponible.

Aperçu rapide pour GLN1 anticorps (all Isoforms) (ABIN2559393)

Antigène: GLN1 (Glutamine Synthetase (GLN1))

Reactivité: Arabidopsis thaliana, Spinach, Zea mays

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLN1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-GLN1 anticorps

Épitope: all Isoforms

Specificité: The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme.

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 39-40 kDa (GLN1,cytoplasmic form), 44-45 kDa (GLN2, chloroplastic form)

Purification: serum

Immunogène: KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1###-1,1-###2,1-###3 and 1-4, (At5g37600, At5g66200, At5g17820, At5g16570)

Information d'application

Indications d'application: Recommended Dilution: 1 : 10000 on 0.5-15 ug protein (WB).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Références pour anticorps anti-GLN1 (ABIN2559393)

Ortigosa, Valderrama-Martín, Ávila, Cánovas, Cañas: "Understanding plant nitrogen nutrition through a laboratory experiment." dans: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, Vol. 47, Issue 4, pp. 450-458, (2019) (PubMed).

Détails sur GLN1

Antigène: GLN1 (Glutamine Synthetase (GLN1))

Autre désignation: GS1 glutamine synthetase

Sujet: AGI Code: At5g37600
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts

Poids moléculaire: 39-40 kDa (GLN1 kDa,cytoplasmic form) , 44-45 kDa (GLN2 kDa, chloroplastic form)

UniProt: Q56WN1, Q9FMD9, Q8LCE1, Q9LVI8

Pathways: Positive Regulation of Peptide Hormone Secretion