ICAM1 anticorps (AA 313-327)

N° du produit ABIN2613438

L’anticorps Chèvre Polyclonal anti-ICAM1 a été validé pour WB, FACS, ELISA et IF. Il convient pour détecter ICAM1 dans des échantillons de Humain.

Aperçu rapide pour ICAM1 anticorps (AA 313-327) (ABIN2613438)

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ICAM1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-ICAM1 anticorps

Épitope: AA 313-327

Fonction: ICAM1 (aa313-327)

Séquence: PNVILTKPEV SEGTE

Specificité: The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite..

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 100 kDa band observed in Human Lung lysates (calculated MW of 57.8 kDa according to Human NP_000192.2).This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells and Human peripheral blood monocytes

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur ICAM1

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: ICAM1

Sujet: ICAM1, intercellular adhesion molecule 1, BB2, CD54, P3.58, ICAM-1, cell surface glycoprotein P3.58, human rhinovirus receptor, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor

ID gène: 3383

NCBI Accession: NP_000192

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling