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Vimentin anticorps (C-Term)

Name: Vimentin anticorps (C-Term)
Brand: antibodies-online
SKU: ABIN2613493
Price: 809.48 EUR
Availability: InStock

Verified VIM Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IF, FACS Hôte: Chèvre Polyclonal unconjugated

ABIN2613493 (0. (Vimentin anticorps (C-Term))

(ABIN2613493) (5 μg/mL) staining of paraffin embedded Human Kidney. (Vimentin anticorps (C-Term))

IHC

ABIN2613493 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). (Vimentin anticorps (C-Term))

ABIN2613493 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (Vimentin anticorps (C-Term))

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0. (Vimentin anticorps (C-Term))

FACS

5 images

N° du produit ABIN2613493

Fiche de données

Antigène Voir toutes Vimentin (VIM) Anticorps

Vimentin (VIM)
Épitope
32

18

16

16

16

11

11

8

7

7

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivité
323

125

122

60

58

43

42

36

30

22

20

14

13

8

7

6

6

3

3

2

1

1

1

1

1

1

1
Humain, Souris, Rat
Hôte
184

123

7

7

6

4

1
Chèvre
Anticorps secondaires appropriés
Clonalité
196

135
Polyclonal
Conjugué
188

19

14

13

10

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
Cet anticorp Vimentin est non-conjugé
Application
271

127

96

96

92

56

43

40

39

28

23

20

8

5

4

4

4

2

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction

Vimentin

Séquence

QVINETSQHH DDLE

Réactivité croisée

Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité

Verified

Isotype

IgG

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Indications d'application

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 55 kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60 kDa band in Rat Ovary lysates (calculated MW of 53.7 kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1).
Peptide ELISA: antibody detection limit dilution 1:4000.

Commentaires

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended conc

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation

Minimize freezing and thawing.

Stock

-20 °C

Stockage commentaire

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Antigène

Vimentin (VIM)

Autre désignation

VIM (VIM Produits)

Synonymes

anticorps CTRCT30, anticorps cb28, anticorps vime, anticorps vim, anticorps vim1, anticorps vim2, anticorps VIM, anticorps Vimentin, anticorps vim4, anticorps vimentin, anticorps vimentin L homeolog, anticorps vimentin S homeolog, anticorps VIM, anticorps Vim, anticorps vim, anticorps vim.L, anticorps vim.S

Sujet

VIM, vimentin, FLJ36605

ID gène

7431, 22352, 81818

NCBI Accession

NP_003371

Pathways

Caspase Cascade in Apoptosis