MADCAM1 anticorps

N° du produit ABIN2625029

Cet anticorps anti-MADCAM1 Monoclonal Souris (Clone 314G8) (ABIN2625029) détecte spécifiquement MADCAM1 dans WB, ELISA, FACS, IHC et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour MADCAM1 anticorps (ABIN2625029)

Antigène: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MADCAM1 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 314G8

Détail du produit anti-MADCAM1 anticorps

Specificité: Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone Reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.

Purification: Protein G purified

niveau d'endotoxine: Low Endotoxin

Immunogène: Recombinant soluble MAdCAM-1-fc fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA, Flo (1:10 - 1:100), IHC, IHC-P (10 μg/mL), WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining. This product does not require antigen retrieval prior to staining of paraffin sections. Western Blot: LS-C44686 detects a band of approximately 60kD. Reducing conditions required. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.

Détails sur MADCAM1

Antigène: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Autre désignation: MADCAM1 / MAdCAM-1

Sujet: Name/Gene ID: MADCAM1

Synonyms: MADCAM1, HMAdCAM-1, MACAM1, MAdCAM-1

ID gène: 8174

UniProt: Q13477