Bovine Coronavirus anticorps

N° du produit ABIN263581

Cet anticorps Souris Monoclonal détecte spécifiquement Bovine Coronavirus dans EIA. Il présente une réactivité avec des échantillons de Bovine Coronavirus (BCoV).

Aperçu rapide pour Bovine Coronavirus anticorps (ABIN263581)

Antigène: Bovine Coronavirus (BCoV)

Reactivité: Bovine Coronavirus (BCoV)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Bovine Coronavirus est non-conjugé

Application: Enzyme Immunoassay (EIA)

Clone: 5A4

Détail du produit anti-Bovine Coronavirus anticorps

Attributs du produit: Synonyms: Spike glycoprotein, S glycoprotein

Purification: Protein G chromatography

Pureté: > 95 % pure (SDS-PAGE)

Immunogène: Bovine corona virus. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/cmice.

Isotype: IgG1

Information d'application

Indications d'application: ELISA. HIT (hemagglutination inhibition titer).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.8mg/mL (OD280nm E0.1%=1.4)

Buffer: PBS, pH 7.4 containing 0.09 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Bovine Coronavirus

Antigène: Bovine Coronavirus (BCoV)

Classe de substances: Virus

Sujet: Bovine Corona Virus Spike glycoprotein is cleaved into S1 and S2 Spike proteins. The precursor is processed into S1 and S2 by host cell furin or furin-like protease to yield the mature S1 and S2 proteins. The cleavage site between S1 and S2 requires the optimal sequence [KR]-X-[KR]-R.S1 attaches the virion to the cell membrane by binding to 9-O-acetylated sialic acid containing proteins, initiating the infection.S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.Synonyms: E2 protein, Peplomer protein, S glycoprotein, Spike glycoprotein

UniProt: P25193