CD69 anticorps

N° du produit ABIN263925

Cet anticorps anti-CD69 est un anticorps Souris Monoclonal détectant CD69 dans FACS, WB, IHC (fro) et Func. Adapté pour Humain. Ce Primary Antibody a été cité dans 6+ publications.

Aperçu rapide pour CD69 anticorps (ABIN263925)

Antigène: CD69

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD69 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: FN50

Détail du produit anti-CD69 anticorps

Specificité: The antibody recognizes CD69, an lymphocyte early activation marker detected as a 53 kDa band under nonreducing and as 29 + 32 kDa double band under reducing SDS-PAGE conditions.

Attributs du produit: Synonyms: GP32/28, Leu-23, MLR-3, EA1, BL-AC/P26, AIM, Early T-cell activation antigen p60, Earlyactivation antigen CD69, Activation inducer molecule, C-type lectin domain family 2member C

Purification: Affinity Chromatography on Protein A

Immunogène: Anti-µ-stimulated human B lymphocytes

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 5 μg/mL. Western blot: 53 kDa, under reducing conditions it shows two bands of 29 and 32 kDa. Immunohistochemistry on Frozen Sections. Functional Assay: When stimulating peripheral blood T cells, FN50 antibody together withTPA synergistically increases cell volume and RNA/DNA synthesis. Addition of FN50 tostimulated peripheral blood B cells has similar, but weaker effect.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS with 15 mM Sodium Azide, pH ~7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Références pour anticorps anti-CD69 (ABIN263925)

Zawada, Mrak, Biedermann, Palmer, Gentleman, Aboud, Griffin et al.: "Loss of angiotensin II receptor expression in dopamine neurons in Parkinson's disease correlates with pathological progression and is accompanied by increases in Nox4- and 8-OH guanosine-related ..." dans: Acta neuropathologica communications, Vol. 3, pp. 9, (2015) (PubMed).

Johnson, Zhang, Adams, Phillips, Freitas, Froehner, Green-Church, Montanaro: "Proteomic analysis reveals new cardiac-specific dystrophin-associated proteins." dans: PLoS ONE, Vol. 7, Issue 8, pp. e43515, (2012) (PubMed).

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." dans: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Krämer, Kess, Kremmer, Schulte-Hostede: "Multi-parameter determination of TNF?, PCT and CRP for point-of-care testing." dans: The Analyst, Vol. 136, Issue 4, pp. 692-5, (2011) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." dans: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Tomescu, Chehimi, Maino, Montaner: "NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2097-104, (2007) (PubMed).

Détails sur CD69

Antigène: CD69

Autre désignation: CD69

Sujet: CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets, it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.Synonyms: AIM, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, Early activation antigen CD69, GP32/28, Leu-23, MLR-3

ID gène: 969

UniProt: Q07108