Nitrotyrosine anticorps

N° du produit ABIN263971

Cet anticorps Souris Monoclonal détecte spécifiquement Nitrotyrosine dans WB, IP, IHC (fro) et EIA. Il présente une réactivité envers .

Aperçu rapide pour Nitrotyrosine anticorps (ABIN263971)

Antigène: Nitrotyrosine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nitrotyrosine est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 39B6

Détail du produit anti-Nitrotyrosine anticorps

Attributs du produit: Synonyms: NO-Tyrosine, Nitro-Tyrosine

Purification: Affinity Chromatography on Protein G.

Immunogène: 3-(4-Hydroxy-3-Nitrophenyl Acetamido) Propionic Acid-BSA conjugate

Isotype: IgG2a

Information d'application

Indications d'application: ELISA (1,6). Immunoprecipitation. Western blot (1): 0.7 μg/mL was sufficient for detection of 5 μg SN-1 treated BSA using Goatanti-Mouse IgG-HRP as the secondary antibody. Immunohistochemistry on Frozen Sections (1,5).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative in 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Nitrotyrosine

Antigène: Nitrotyrosine

Classe de substances: Chemical

Sujet: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an mportant component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, sinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high orrelation to disease states such as atherosclerosis, Alzheimer' disease, Parkinson's disease and amyotrophic lateral sclerosis (4).Synonyms: NO-Tyrosine, Nitro-Tyrosine