CD2 anticorps (APC)

N° du produit ABIN264177

Cet anticorps anti-CD2 est un anticorps Souris Monoclonal détectant CD2 dans FACS. Adapté pour Humain.

Aperçu rapide pour CD2 anticorps (APC) (ABIN264177)

Antigène: CD2

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD2 est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Clone: T6-3

Détail du produit anti-CD2 anticorps (APC)

Attributs du produit: Synonyms: T11/Leu-5, LFA-2, LFA-3 receptor, Erythrocyte receptor, Rosette receptor, SRBC, T-cellsurface antigen CD2, T-cell surface antigen T11/Leu-5

Immunogène: Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/cmice immunized with T Lymphocytes activated by mixed lymphocyte culture.

Isotype: IgG2a

Information d'application

Indications d'application: Suitable for Monitoring of T cells in peripheral blood, Analysis of NK subsets, Study of Tcell activation. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: PBMC: Add 10 ul of Anitbody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of Antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at roomtemperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturer's instructions for Lysed Whole Blood andImmunofluorescence analysis with a Flow cytometer or microscope.

Restrictions: For Research Use only

Stockage

Buffer: PBS with 0.08 % sodium azide as preservative and 0.2 % protein carrier.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody (undiluted) at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur CD2

Antigène: CD2

Autre désignation: CD2

Sujet: Identification of human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.Synonyms: Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, SRBC, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5

UniProt: P06729