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Calpain anticorps

Cet anticorps anti-Calpain est un anticorps Souris Monoclonal détectant Calpain dans WB, IP et EIA. Adapté pour Humain et Boeuf (Vache).
N° du produit ABIN264387

Aperçu rapide pour Calpain anticorps (ABIN264387)

Antigène

Calpain

Reactivité

  • 4
  • 3
  • 1
  • 1
Humain, Boeuf (Vache)

Hôte

  • 4
  • 3
Souris

Clonalité

  • 4
  • 3
Monoclonal

Conjugué

  • 4
  • 1
  • 1
  • 1
Cet anticorp Calpain est non-conjugé

Application

  • 6
  • 4
  • 3
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone

28F3
  • Attributs du produit

    Synonyms: CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1

    Purification

    Standard chromatographic techniques.

    Immunogène

    Calpain purified from human placenta.

    Isotype

    IgG1
  • Indications d'application

    ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Buffer

    0.02 M Sodium Phosphate, pH 7.5, 0.15 M Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as presevtative.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    -20 °C

    Stockage commentaire

    Store the antibody at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.

    Date de péremption

    12 months
  • Antigène

    Calpain

    Sujet

    The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP-2, CANP1, CANPL1, CANPL2, CAPN1, CAPN2
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