Myogenin anticorps (AA 30-224)

N° du produit ABIN264431

L’anticorps Souris Monoclonal anti-Myogenin a été validé pour WB, IF, IP, IHC (p), EIA et EMSA. Il convient pour détecter Myogenin dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Myogenin anticorps (AA 30-224) (ABIN264431)

Antigène: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Myogenin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: F12B

Détail du produit anti-Myogenin anticorps

Épitope: AA 30-224

Specificité: Recognizes a protein of 34 kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin. This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.

Attributs du produit: Synonyms: MYOG, MYF4, Myogenic factor 4, BHLHC3, Class C basic helix-loop-helix protein 3

Purification: Purified

Immunogène: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withrecombinant protein containing rat myogenin amino acids 30-224 and mouse myelomaNS1 cells. Genename: MYOG

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Gel Supershift. Immunohistochemistry on Formalin-fixed, Paraffin-embedded tissue sections (1-2 μg/mL). Immunofluorescence. Immunoprecipitation (2 μg/mL). Western Blot (1 μg/mL). Positive Control: Rhabdomyosarcoma and Wlim's tumors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur Myogenin

Antigène: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Autre désignation: Myogenin

Sujet: Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.Synonyms: BHLHC3, Class C basic helix-loop-helix protein 3, MYF4, MYOG, Myogenic factor 4

ID gène: 29148

UniProt: P20428

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development