Myogenin anticorps (AA 30-224)

N° du produit ABIN264431

L’anticorps anti-Myogenin Monoclonal Souris est utilisé pour la détection de Myogenin dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IF, IP, IHC (p), EIA et EMSA.

Aperçu rapide pour Myogenin anticorps (AA 30-224) (ABIN264431)

Antigène: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Myogenin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: F12B

Détail du produit anti-Myogenin anticorps

Épitope: AA 30-224

Specificité: Recognizes a protein of 34 kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin. This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.

Attributs du produit: Synonyms: MYOG, MYF4, Myogenic factor 4, BHLHC3, Class C basic helix-loop-helix protein 3

Purification: Purified

Immunogène: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withrecombinant protein containing rat myogenin amino acids 30-224 and mouse myelomaNS1 cells. Genename: MYOG

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Gel Supershift. Immunohistochemistry on Formalin-fixed, Paraffin-embedded tissue sections (1-2 μg/mL). Immunofluorescence. Immunoprecipitation (2 μg/mL). Western Blot (1 μg/mL). Positive Control: Rhabdomyosarcoma and Wlim's tumors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur Myogenin

Antigène: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Autre désignation: Myogenin

Sujet: Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.Synonyms: BHLHC3, Class C basic helix-loop-helix protein 3, MYF4, MYOG, Myogenic factor 4

ID gène: 29148

UniProt: P20428

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development