GFP anticorps

N° du produit ABIN264457

Cet anticorps Souris Monoclonal détecte spécifiquement GFP dans WB. Il présente une réactivité envers Aequorea victoria.

Aperçu rapide pour GFP anticorps (ABIN264457)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB)

Clone: F56-BA1-2-3

Détail du produit anti-GFP anticorps

Attributs du produit: Synonyms: Green fluorescent protein, GFP-Tag

Purification: Purified

Immunogène: Hybridoma produced by the fusion of splenocytes of mice immunized with full-length greenfluorescent protein and mouse myeloma cells.

Isotype: IgG

Information d'application

Indications d'application: Western Blot (5-10 μg/mL). Positive Control: Purified Green Fluorescent Protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing containing 0.08 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein

UniProt: P42212