Cet anticorps anti-DNA Adenine Methylase Monoclonal Souris (Clone RB1) (ABIN264462) détecte spécifiquement DNA Adenine Methylase dans WB.
L’anticorps est réactif avec des échantillons de Bactéries.
Western blot (5-10 μg/mL). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 0.08 % Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Date de péremption
12 months
Antigène
DNA Adenine Methylase
Sujet
DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence), (ii) methyl-directed mismatch repair, (iii) control of transposition of certain transposable DNA elements, and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science, 284:967-970, 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent.Synonyms: DAM, DNA adenine methyltransferase, Deoxyadenosyl-methyltransferase