Nitrotyrosine anticorps

N° du produit ABIN264465

Cet anticorps anti-Nitrotyrosine Monoclonal Souris (Clone 2A12) (ABIN264465) détecte spécifiquement Nitrotyrosine dans WB et IHC (fro).

Aperçu rapide pour Nitrotyrosine anticorps (ABIN264465)

Antigène: Nitrotyrosine

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nitrotyrosine est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2A12

Détail du produit anti-Nitrotyrosine anticorps

Attributs du produit: Synonyms: NO-Tyrosine, Nitro-Tyrosine

Purification: Purified

Immunogène: 3-Nitrotyrosine-KLH

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: 1/1000. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: 20 mM Sodium Phosphate, 150 mM Sodium Chloride, 50 % Glycerol, 3 mM Sodium Azide, pH 7.5

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody can be shipped at 2-8 °C. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Nitrotyrosine

Antigène: Nitrotyrosine

Classe de substances: Chemical

Sujet: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxidesignaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis.55 kD 160 kD.Synonyms: NO-Tyrosine, Nitro-Tyrosine