SOD1 anticorps

N° du produit ABIN264854

Il existe 3+ publications pour ce produit. L’anticorps anti-SOD1 Polyclonal Lapin est utilisé pour la détection de SOD1 dans des échantillons de Humain, Souris, Rat et Boeuf (Vache). Il a été validé pour WB, IP, IHC (fro) et EIA.

Aperçu rapide pour SOD1 anticorps (ABIN264854)

Antigène: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SOD1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Détail du produit anti-SOD1 anticorps

Specificité: Detects a 23 kDa (Human) and 19 kDa (other species) proteins corresponding to the Molecular Mass of Cu/Zn Superoxide Dismutase (SOD) on SDS-PAGE Immunoblots.

Purification: Antigen Affinity Chromatography

Immunogène: Rat Cu/Zn SOD

Isotype: IgG

Information d'application

Indications d'application: Western blot: 0.5 μg/mL was sufficient for detection of Cu/Zn SOD in 20 μg of rat braintissue extract by Colorimetric Immunoblot analysis using Goat anti-RabbitIgG-AP as the secondary antibody. (Ref.6)Immunoprecipitation. EIA (Ref.6). Immunohistochemistry on Frozen Sections (Ref.8).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.0, 0.09 % Sodium Azide, 50 % Glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-SOD1 (ABIN264854)

Zuber, Rappaport, Luo, Wang, Chen, Vaseva, Shi, Weissmueller, Fellmann, Fellman, Taylor, Weissenboeck, Graeber, Kogan, Vakoc, Lowe: "An integrated approach to dissecting oncogene addiction implicates a Myb-coordinated self-renewal program as essential for leukemia maintenance." dans: Genes & development, Vol. 25, Issue 15, pp. 1628-40, (2011) (PubMed).

Wang, Kumagai-Braesch, Herrington, Larsson, Permert: "Increased lipid metabolism and cell turnover of MiaPaCa2 cells induced by high-fat diet in an orthotopic system." dans: Metabolism: clinical and experimental, Vol. 58, Issue 8, pp. 1131-6, (2009) (PubMed).

Huang, Lee, Kuo, Yang, Chien: "Attenuation of long-term Rhodiola rosea supplementation on exhaustive swimming-evoked oxidative stress in the rat." dans: The Chinese journal of physiology, Vol. 52, Issue 5, pp. 316-24, (2009) (PubMed).

Détails sur SOD1

Antigène: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Autre désignation: Superoxide Dismutase 1 / SOD1

Sujet: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (Ref.3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (Ref.2,5). In general, SODs play a major role in antioxidant defense mechanisms. (Ref.4) There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (Ref.3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (Ref.7). SOD3 can also be distinguished by its heparinbinding capacity (Ref.1).Synonyms: ALS1, CuZn-SOD, CuZnSOD, IPOA, SOD-1, Superoxide dismutase [Cu-Zn]

ID gène: 6647

NCBI Accession: NP_000445

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis