SOD1 anticorps

N° du produit ABIN264856

Cet anticorps Lapin Polyclonal détecte spécifiquement SOD1 dans WB, IP, IF, IHC (fro) et EIA. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Lapin, Mouton, Porc, Singe, Chien, Hamster, Corail et Xenopus laevis.

Aperçu rapide pour SOD1 anticorps (ABIN264856)

Antigène: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin, Mouton, Porc, Singe, Chien, Hamster, Corail, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SOD1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Détail du produit anti-SOD1 anticorps

Specificité: Detects 23 kDa (Human) and 19 kDa (Other species) proteins, corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS-PAGE immunoblots.

Purification: Antigen Affinity Chromatography

Immunogène: Human Cu/Zn SOD

Isotype: IgG

Information d'application

Indications d'application: Western blot (6,8): 0.2 μg/mL was sufficient for detection of Cu/Zn SOD in 20 g of HeLacell lysate. Immunoprecipitation (8). EIA (6). Immunohistochemistry on frozen sections(7).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS ( pH 7.0), 0.09 % Sodium Azide, 50 % Glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur SOD1

Antigène: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Autre désignation: Superoxide Dismutase 1 / SOD1

Sujet: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2¯ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(9). SOD3 can also be distinguished by its heparin-binding capacity (1).Synonyms: ALS1, CuZn-SOD, CuZnSOD, IPOA, SOD-1, Superoxide dismutase [Cu-Zn]

ID gène: 6647

NCBI Accession: NP_000445

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis