S1PR1 anticorps (C-Term)

N° du produit ABIN264982

Détail du produit anti-S1PR1 anticorps

Antigène: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp S1PR1 est non-conjugé

Application: Western Blotting (WB)

Specificité: This antibody reacts to EDG1.

Purification: Purified

Immunogène: Synthetic peptide derived from the C-terminal of the EDG-1 receptor

Isotype: IgG

Information d'application

Indications d'application: Western Blot: 5-10 μg/mL, Detects an approximately 44 kDa band in RH7777 cellstransfected with full length human EDG-1. Due to low expression of EDG receptors, werecommend use of Pierce Femto Signal substrate for western blot development.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Références pour anticorps anti-S1PR1 (ABIN264982)

Zhang, Loyd, Randall, Waddell, Kriwacki, Ney: "A short linear motif in BNIP3L (NIX) mediates mitochondrial clearance in reticulocytes." dans: Autophagy, Vol. 8, Issue 9, pp. 1325-32, (2013) (PubMed).

Goparaju, Jolly, Watterson, Bektas, Alvarez, Sarkar, Mel, Ishii, Chun, Milstien, Spiegel: "The S1P2 receptor negatively regulates platelet-derived growth factor-induced motility and proliferation." dans: Molecular and cellular biology, Vol. 25, Issue 10, pp. 4237-49, (2005) (PubMed).

Matsushita, Morrell, Lowenstein: "Sphingosine 1-phosphate activates Weibel-Palade body exocytosis." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 31, pp. 11483-7, (2004) (PubMed).

Toman, Payne, Watterson, Maceyka, Lee, Milstien, Bigbee, Spiegel: "Differential transactivation of sphingosine-1-phosphate receptors modulates NGF-induced neurite extension." dans: The Journal of cell biology, Vol. 166, Issue 3, pp. 381-92, (2004) (PubMed).

Yamaguchi, Kitayama, Takuwa, Arikawa, Inoki, Takehara, Nagawa, Takuwa: "Sphingosine-1-phosphate receptor subtype-specific positive and negative regulation of Rac and haematogenous metastasis of melanoma cells." dans: The Biochemical journal, Vol. 374, Issue Pt 3, pp. 715-22, (2003) (PubMed).

Cummings, Parinandi, Zaiman, Wang, Usatyuk, Garcia, Natarajan: "Phospholipase D activation by sphingosine 1-phosphate regulates interleukin-8 secretion in human bronchial epithelial cells." dans: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 30227-35, (2002) (PubMed).

Graeler, Goetzl: "Activation-regulated expression and chemotactic function of sphingosine 1-phosphate receptors in mouse splenic T cells." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 16, Issue 14, pp. 1874-8, (2002) (PubMed).

Détails sur S1PR1

Antigène: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Autre désignation: EDG-1 / S1PR1 (S1PR1 Produits)

Synonymes: anticorps CD363, anticorps CHEDG1, anticorps D1S3362, anticorps ECGF1, anticorps EDG-1, anticorps EDG1, anticorps S1P1, anticorps AI849002, anticorps Edg1, anticorps Lpb1, anticorps S1p, anticorps S1p1, anticorps S1P receptor 1, anticorps S1P receptor Edg-1, anticorps edg1, anticorps etID43307.22, anticorps s1p1, anticorps wu:fb98f05, anticorps s1pr1, anticorps xts1p1, anticorps sphingosine-1-phosphate receptor 1, anticorps sphingosine-1-phosphate receptor 1 L homeolog, anticorps S1PR1, anticorps S1pr1, anticorps s1pr1, anticorps s1pr1.L

Sujet: EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocytecontaining and other tissues.Synonyms: CHEDG1, D1S3362, ECGF1, EDG1, Endothelial differentiation G-protein coupled receptor 1, FLJ58121, S1P receptor 1, S1PR1, Sphingosine 1-phosphate receptor 1

ID gène: 1901

NCBI Accession: NP_001391

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2