SPARC anticorps

N° du produit ABIN265180

Cet anticorps Lapin Polyclonal détecte spécifiquement SPARC dans WB, IHC (fro) et EIA. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour SPARC anticorps (ABIN265180)

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SPARC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Détail du produit anti-SPARC anticorps

Specificité: This antibody detects SPARC. It may Cross react with SPARCL1.

Purification: Ammonium Sulfate Precipitation

Immunogène: Synthetic peptide derived from the Human SPARC protein

Isotype: IgG

Information d'application

Indications d'application: Western blot: (1-5 μg/mL). ELISA: Immunohistochemistry on Frozen Sections. Positive Control/Tissue Expression: Mouse heart lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur SPARC

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Autre désignation: SPARC / Osteonectin

Sujet: SPARC is a key factor in cell-matrix interactions and possibly tumour aggressiveness. The SPARC gene, which encodes a multifunctional glycoprotein with roles in tissue development, remodelling and fibrosis. A regulator of cell-extracellular matrix (ECM) interactions, SPARC represents a major factor in the ECM remodelling occurring during tumour invasion. in silico analysis reveals 4 UTR-SNPs located in the 3 -UTR of the SPARC gene, corresponding to 1474 g a, 1551 g c, 1922 t g and 2072 c t changes, which are significantly associated with tumoral state of the tissue. Of all hits, the 2072 SPARC polymorphism had the best association with cancer. SPARC therefore is a gene involved in a number of diseases including rheumatoid arthritis, scleroderma, tumor development and metastasis. SPARC variants have been detected in tumour samples of patients with acute myeloblastic leukemia (AML).Synonyms: BM-40, Basement-membrane protein 40, ON, Secreted protein acidic and rich in cysteine

ID gène: 6678

NCBI Accession: NP_003109

UniProt: P09486

Pathways: Autophagy