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Cyclin F anticorps

Cet anticorps Lapin Polyclonal détecte spécifiquement Cyclin F dans WB, IHC (p) et EIA. Il présente une réactivité envers Humain.
N° du produit ABIN265381

Aperçu rapide pour Cyclin F anticorps (ABIN265381)

Antigène

Voir toutes Cyclin F (CCNF) Anticorps
Cyclin F (CCNF)

Reactivité

  • 43
  • 16
  • 8
Humain

Hôte

  • 40
  • 3
Lapin

Clonalité

  • 41
  • 2
Polyclonal

Conjugué

  • 14
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Cyclin F est non-conjugé

Application

  • 33
  • 17
  • 13
  • 13
  • 5
  • 3
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Specificité

    This antibody detects endogenous levels of Cyclin F protein (region surrounding Ile772).

    Réactivité croisée (Details)

    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.

    Purification

    The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

    Pureté

    > 95 % pure by SDS-PAGE
  • Indications d'application

    ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    Cyclin F (CCNF)

    Autre désignation

    Cyclin F

    Sujet

    Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.Synonyms: CCNF, Cyclin-F, G2/mitotic-specific cyclin-F

    Poids moléculaire

    approx. 88 kDa

    ID gène

    899

    NCBI Accession

    NP_001752

    UniProt

    P41002
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