Factor VIII anticorps

N° du produit ABIN265412

L’anticorps anti-Factor VIII Polyclonal Lapin est utilisé pour la détection de Factor VIII dans des échantillons de Humain et Souris. Il a été validé pour WB et IHC (p).

Aperçu rapide pour Factor VIII anticorps (ABIN265412)

Antigène: Factor VIII (F8) (Coagulation Factor VIII (F8))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Factor VIII est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Factor VIII anticorps

Specificité: This antibody detects endogenous levels of Coagulation factor VIII (F8) protein (region surronding Ser2194).

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Factor VIII

Antigène: Factor VIII (F8) (Coagulation Factor VIII (F8))

Sujet: Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin, and Factors X, IX, V, and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation Factor VII (serum prothrombin conversion accelerator, proconvertin, F7, Factor VII) is a 406 amino acid, vitamin K-dependent, single chain serine protease that is synthesized in the liver and circulates as an inactive precursor. Factor IX A, Factor X A, Factor XII A, or thrombin mediated proteolytic cleavage of Factor VII at Arg152-Ile153 generates Factor VII A, an active serine protease composed of a catalytic heavy chain disulfide linked to a light chain, containing 2 EGF-like domains. Mutations at the F7 locus that lead to Factor VII deficiencies are generally asymptomatic or phenotypically uncharacterized, with hemorrhagic diathesis occurring at extremely low levels.Synonyms: AHF, Antihemophilic factor, F8C, Procoagulant component

Poids moléculaire: approx. 267 kDa

ID gène: 2157

NCBI Accession: NP_000123

UniProt: P00451