Recombinant ART2.2/Art2b anticorps

N° du produit ABIN2656684

L’anticorps Souris Monoclonal anti-ART2.2/Art2b a été validé pour ICFC. Il convient pour détecter ART2.2/Art2b dans des échantillons de Souris.

Aperçu rapide pour Recombinant ART2.2/Art2b anticorps (ABIN2656684)

Antigène: ART2.2/Art2b (Art2b) (ADP-Ribosyltransferase 2b (Art2b))

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ART2.2/Art2b est non-conjugé

Application: Intracellular Flow Cytometry (ICFC)

Clone: S-16a

Détail du produit anti-ART2.2/Art2b anticorps

Marque: Treg-Protector™

Attributs du produit: ARTC2, also known as ART2.2, is a 35 kD, GPI-anchored, and cell surface ADP-ribosyltransferase that is highly expressed by regulatory T cells (Tregs) and natural killer T (NKT) cells. ARTC2 is enzymatically active at 4°C. Through the ribosylation of the ion channel P2X7, ARTC2 mediates NAD+-induced cell death (NICD) and the proteolytic shedding of CD27 and CD62L. Blocking the activity ARTC2 with Treg-Protector™ improves the recovery of Tregs and NKT cells during their isolation from lymphatic and peripheral tissues.

Pureté: ≥ 95%, as determined by Coomassie stained SDS-PAGE.

niveau d'endotoxine: Less than 0.01 ng of endotoxin/µg of protein, as determined by the LAL method.

Immunogène: Expression vector encoding full length mouse ARTC2.

Isotype: Ig Fraction

Information d'application

Indications d'application: Treg-Protector™ (S+16a) is a recombinant llama VH Ig domain that binds to the ADP-ribosyltransferase ARTC2, which reversibly blocks its enzymatic activity and protects ARTC2+ cells from NAD+-induced cell death (NICD), which is caused by the release of NAD+ by cells that die during the preparation of primary lymphocytes. The S+16a has been demonstrated to protect a variety of cell types including Tregs, NKT cells, and tissue-resident memory T cells.

Commentaires:

We recommend diluting Treg-Protector™ 1:4 with sterile PBS and administer 200 µL (50 µg) intravenously (i.v.) of diluted Treg-Protector™ per mouse. Euthanize the mouse and begin the cell preparation 15 - 60 minutes after the injection. Intraperitoneal (IP) injection is also effective at similar doses (validated by developer).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: 1 mg/mL S-16a in PBS Treg-Protector ™ is sterilized by filtration through a 0.22 μm membrane and contains no preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Do not freeze. Keep sterile.

Stock: 4 °C

Stockage commentaire: Store between 2°C and 8°C. Do not freeze. Keep sterile.

Détails sur ART2.2/Art2b

Antigène: ART2.2/Art2b (Art2b) (ADP-Ribosyltransferase 2b (Art2b))

Autre désignation: ARTC2 Nanobody

ID gène: 11872