DNMT3A anticorps

N° du produit ABIN2668262

Cité dans 3 publications. Cet anticorps anti-DNMT3A Monoclonal Souris (Clone 64B1446) (ABIN2668262) détecte spécifiquement DNMT3A dans WB, IHC et ChIP. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour DNMT3A anticorps (ABIN2668262)

Antigène: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DNMT3A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)

Clone: 64B1446

Détail du produit anti-DNMT3A anticorps

Fonction: DNMT3A antibody (mAb) (Clone 64B1446)

Purification: Protein G Chromatography

Immunogène: This DNMT3A antibody was raised against a full-length protein corresponding to mouse DNMT3A.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Validated Applications: WB: 2 - 4 μg/mL dilution Individual optimization may be required. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. Published Applications: ChIP IHC See references for more information.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 μg/μL

Buffer: PBS containing 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: 4 °C,-20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-DNMT3A (ABIN2668262)

Petell, Alabdi, He, San Miguel, Rose, Gowher: "An epigenetic switch regulates de novo DNA methylation at a subset of pluripotency gene enhancers during embryonic stem cell differentiation." dans: Nucleic acids research, Vol. 44, Issue 16, pp. 7605-17, (2016) (PubMed).

Karius, Schnekenburger, Ghelfi, Walter, Dicato, Diederich: "Reversible epigenetic fingerprint-mediated glutathione-S-transferase P1 gene silencing in human leukemia cell lines." dans: Biochemical pharmacology, Vol. 81, Issue 11, pp. 1329-42, (2011) (PubMed).

Saito, Kato, Hiraoka, Horii, Suzuki, Higashi, Kaji, Kondo, Yamamoto: "DNA methylation of colon mucosa in ulcerative colitis patients: correlation with inflammatory status." dans: Inflammatory bowel diseases, Vol. 17, Issue 9, pp. 1955-65, (2011) (PubMed).

Détails sur DNMT3A

Antigène: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Autre désignation: DNMT3A

Sujet: DNMT3A - DNA methyltransferase proteins such as DNMT3A (or DNMT 3A) are involved in DNA methylation, in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. DNMT3A has been shown to be important in the regulation of specific patterns of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression. Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family, containing two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L), establishes the initial CpG methylation pattern de novo and shows the same propensity for methylating unmethylated duplex DNA as for hemi-methylated DNA. DNMT3A and DNMT3B are encoded by two distinct genes and are strongly expressed in embryonic stem cells. DNMT3A is ubiquitous in mammalian cells.

Poids moléculaire: 116 kDa

ID gène: 1788

NCBI Accession: NP_001258682