Histone H2A, Idic Patch (AA 89-91) anticorps

N° du produit ABIN2668306

Il existe 4+ publications pour ce produit. L’anticorps anti-Histone H2A, Idic Patch Polyclonal Lapin est utilisé pour la détection de Histone H2A, Idic Patch dans des échantillons de Humain. Il a été validé pour ChIP et WB.

Aperçu rapide pour Histone H2A, Idic Patch (AA 89-91) anticorps (ABIN2668306)

Antigène: Histone H2A, Idic Patch

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Application: Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)

Détail du produit

Épitope: AA 89-91

Fonction: Histone H2A, acidic patch antibody (pAb)

Purification: Unpurified

Immunogène: This Histone H2A, acidic patch antibody was raised against a peptide including amino acids 89-91 of histone H2A.

Information d'application

Indications d'application: ChIP: 10 µL per ChIP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Référence

Lodhi, Kossenkov, Tulin: "Bookmarking promoters in mitotic chromatin: poly(ADP-ribose)polymerase-1 as an epigenetic mark." dans: Nucleic acids research, Vol. 42, Issue 11, pp. 7028-38, (2014) (PubMed).

Alvarez-Saavedra, De Repentigny, Lagali, Raghu Ram, Yan, Hashem, Ivanochko, Huh, Yang, Mears, Todd, Corcoran, Bassett, Tokarew, Kokavec, Majumder, Ioshikhes, Wallace, Kothary, Meshorer, Stopka et al.: "Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation. ..." dans: Nature communications, Vol. 5, pp. 4181, (2014) (PubMed).

Armour, Bennett, Braun, Zhang, McMahon, Gygi, Harper, Sinclair: "A high-confidence interaction map identifies SIRT1 as a mediator of acetylation of USP22 and the SAGA coactivator complex." dans: Molecular and cellular biology, Vol. 33, Issue 8, pp. 1487-502, (2013) (PubMed).

Benatti, Dolfini, Viganò, Ravo, Weisz, Imbriano: "Specific inhibition of NF-Y subunits triggers different cell proliferation defects." dans: Nucleic acids research, Vol. 39, Issue 13, pp. 5356-68, (2011) (PubMed).

Détail du antigène

Antigène: Histone H2A, Idic Patch

Sujet: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.

Poids moléculaire: 14 kDa

ID gène: 92815

NCBI Accession: NP_003508