Histone H2B anticorps (AA 111-125)

N° du produit ABIN2668332

Cet anticorps Lapin Polyclonal détecte spécifiquement Histone H2B dans WB. Il présente une réactivité avec des échantillons de Humain.Il a été cité dans 1 publication.

Aperçu rapide pour Histone H2B anticorps (AA 111-125) (ABIN2668332)

Antigène: Histone H2B

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H2B est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Histone H2B anticorps

Épitope: AA 111-125

Fonction: Histone H2B antibody (pAb)

Purification: Protein G Chromatography

Immunogène: This Histone H2B antibody was raised against a peptide corresponding to amino acid residues 111-125 of human Histone H2B.

Isotype: IgG

Information d'application

Indications d'application: WB: 2 μg/mL dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 μg/μL

Buffer: PBS containing 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: 4 °C,-20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone H2B (ABIN2668332)

Chandrasekharan, Huang, Chen, Sun: "Histone H2B C-terminal helix mediates trans-histone H3K4 methylation independent of H2B ubiquitination." dans: Molecular and cellular biology, Vol. 30, Issue 13, pp. 3216-32, (2010) (PubMed).

Détails sur Histone H2B

Antigène: Histone H2B

Sujet: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.

Poids moléculaire: 15 kDa

ID gène: 8348

NCBI Accession: NP_778225