Histone H2B anticorps (H2BK5ac)

N° du produit ABIN2668338

Cet anticorps Lapin Polyclonal détecte spécifiquement Histone H2B dans WB, IF, ChIP, DB et ChIP-seq. Il présente une réactivité avec des échantillons de Humain.Il a été cité dans 1 publication.

Aperçu rapide pour Histone H2B anticorps (H2BK5ac) (ABIN2668338)

Antigène: Histone H2B

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H2B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Détail du produit anti-Histone H2B anticorps

Épitope: H2BK5ac

Fonction: Histone H2BK5ac antibody (pAb)

Purification: Unpurified

Immunogène: This Histone H2B acetyl Lys5 antibody was raised against a peptide including acetyl-lysine 5 of human histone H2B.

Information d'application

Indications d'application: ChIP: 5 - 10 µL per ChIP WB*: 1:2,000 - 1:10,000 dilution IF: 1:500 dilution The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H2B acetyl Lys5 antibody was validated for ChIP-Seq in the study (see reference). *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone H2B (ABIN2668338)

Egelhofer, Minoda, Klugman, Lee, Kolasinska-Zwierz, Alekseyenko, Cheung, Day, Gadel, Gorchakov, Gu, Kharchenko, Kuan, Latorre, Linder-Basso, Luu, Ngo, Perry, Rechtsteiner, Riddle, Schwartz, Shanower et al.: "An assessment of histone-modification antibody quality. ..." dans: Nature structural & molecular biology, Vol. 18, Issue 1, pp. 91-3, (2011) (PubMed).

Détails sur Histone H2B

Antigène: Histone H2B

Sujet: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription.

Poids moléculaire: 15 kDa

ID gène: 8348

NCBI Accession: NP_778225