Histone 3 anticorps (H3K56ac)

N° du produit ABIN2668471

Il existe 7+ publications pour ce produit. L’anticorps anti-Histone 3 Polyclonal Lapin est utilisé pour la détection de Histone 3 dans des échantillons de Humain et Saccharomyces cerevisiae. Il a été validé pour WB, ChIP, DB, ChIP-seq et CUT&Tag.

Aperçu rapide pour Histone 3 anticorps (H3K56ac) (ABIN2668471)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Détail du produit anti-Histone 3 anticorps

Épitope: H3K56ac

Fonction: Histone H3K56ac antibody (pAb)

Purification: Affinity Purified

Immunogène: This Histone H3 acetyl Lys56 antibody was raised against peptide containing acetyl-Lys56 of yeast histone H3.

Isotype: IgG

Information d'application

Indications d'application: ChIP: 3 - 5 µL per ChIP ChIP-Seq: 10 µL each WB: 1:2,500 - 1:5,000 dilution CUT&Tag: 1-2 µL per 50 µL reaction For Histone H3K56ac, we also offer AbFlex Histone H3K56ac Recombinant Antibody (rAb). For details, see Catalog No. 91127.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.7 μg/μL

Buffer: Purified rabbit IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone 3 (ABIN2668471)

Meisenberg, Ashour, El-Shafie, Liao, Hodgson, Pilborough, Khurram, Downs, Ward, El-Khamisy: "Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan." dans: Nucleic acids research, Vol. 45, Issue 3, pp. 1159-1176, (2017) (PubMed).

Tomazou, Sheffield, Schmidl, Schuster, Schönegger, Datlinger, Kubicek, Bock, Kovar: "Epigenome mapping reveals distinct modes of gene regulation and widespread enhancer reprogramming by the oncogenic fusion protein EWS-FLI1." dans: Cell reports, Vol. 10, Issue 7, pp. 1082-95, (2015) (PubMed).

Yin, Lan, Zhang, Zhu, Chen, Xu, Liu, Ye, Sun, Lu, Zhang: "LSD1 regulates pluripotency of embryonic stem/carcinoma cells through histone deacetylase 1-mediated deacetylation of histone H4 at lysine 16." dans: Molecular and cellular biology, Vol. 34, Issue 2, pp. 158-79, (2013) (PubMed).

Sims, Wade: "Mi-2/NuRD complex function is required for normal S phase progression and assembly of pericentric heterochromatin." dans: Molecular biology of the cell, Vol. 22, Issue 17, pp. 3094-102, (2011) (PubMed).

Foster, Dovey, Lezina, Luo, Gant, Barlev, Bradley, Cowley: "Lysine-specific demethylase 1 regulates the embryonic transcriptome and CoREST stability." dans: Molecular and cellular biology, Vol. 30, Issue 20, pp. 4851-63, (2010) (PubMed).

Dovey, Foster, Cowley: "Histone deacetylase 1 (HDAC1), but not HDAC2, controls embryonic stem cell differentiation." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 18, pp. 8242-7, (2010) (PubMed).

Koch, Pillus: "The glucanosyltransferase Gas1 functions in transcriptional silencing." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 27, pp. 11224-9, (2009) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histones is often associated with transcriptional activation. Histone H3 Lys56 acetylation occurs normally during S phase, but disappears in G2. This modification persists in presence of DNA damage and also plays a role in nucleosome assembly. Rtt109 was shown to be the major histone acetyltransferase (HAT) for Lys56 acetylation.

Poids moléculaire: 17 kDa

ID gène: 3020

NCBI Accession: NP_009564