Histone H3.X/Y anticorps

N° du produit ABIN2668484

Cité dans 1 publication. Cet anticorps anti-Histone H3.X/Y Monoclonal Rat (Clone 8H6-2111) (ABIN2668484) détecte spécifiquement Histone H3.X/Y dans ChIP, ICC, IF et WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Histone H3.X/Y anticorps (ABIN2668484)

Antigène: Histone H3.X/Y

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Application: Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Clone: 8H6-2111

Détail du produit

Fonction: Histone H3.X/Y antibody (mAb) (Clone 8H6-2111)

Purification: Protein G Chromatography

Immunogène: This H3.X/Y antibody was raised against a synthetic peptide corresponding to amino acids 9 - 20 of human H3.X.

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified rat IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Référence

Wiedemann, Mildner, Bönisch, Israel, Maiser, Matheisl, Straub, Merkl, Leonhardt, Kremmer, Schermelleh, Hake: "Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y." dans: The Journal of cell biology, Vol. 190, Issue 5, pp. 777-91, (2010) (PubMed).

Détail du antigène

Antigène: Histone H3.X/Y

Sujet: Histones H3.X and H3.Y are primate-specific variants of histone H3. H3.X and H3.Y are roughly 80 % conserved compared to histone H3.3 The H3.Y protein is highly expressed in several primary cell lines, cancer cell lines and human brain. Expression of histone H3.Y is induced by stress. Exogenously expressed H3.X and H3.Y are efficiently incorporated into chromatin and exhibit dynamics similar to canonical histone H3. Knockdown of H3.Y results in slowed growth and altered expression of cell cycle related genes.

Poids moléculaire: 17 kDa

ID gène: 440093