Rpb1 CTD anticorps (pSer5)

N° du produit ABIN2668520

Cité dans 3 publications. Cet anticorps anti-Rpb1 CTD Polyclonal Lapin (ABIN2668520) détecte spécifiquement Rpb1 CTD dans WB, ChIP et ChIP-seq. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour Rpb1 CTD anticorps (pSer5) (ABIN2668520)

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Rpb1 CTD est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Détail du produit anti-Rpb1 CTD anticorps

Épitope: pSer5

Fonction: RNA pol II CTD phospho Ser5 antibody (pAb)

Purification: Protein A Chromatography

Immunogène: This RNA pol II CTD phospho Ser5 antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at serine 5.

Isotype: IgG

Information d'application

Indications d'application: ChIP: 4 - 10 μg per ChIP ChIP-Seq: 4 µL each WB: 0.5 - 2 μg/mL dilution For RNA pol II CTD phospho Ser5, we also offer AbFlex RNA pol II CTD phospho Ser5 Recombinant Antibody (rAb). For details, see Catalog No. 91119.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Rpb1 CTD (ABIN2668520)

Young, Hillyer, Hokamp, Fitzpatrick, Konstantinov, Welty, Ness, Werner-Washburne, Fleming, Osley: "Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast." dans: BMC genomics, Vol. 18, Issue 1, pp. 107, (2017) (PubMed).

Ke, McKnight, Gracey Maniar, Sun, Callaway, Majnik, Lane, Cohen: "IUGR increases chromatin-remodeling factor Brg1 expression and binding to GR exon 1.7 promoter in newborn male rat hippocampus." dans: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 309, Issue 2, pp. R119-27, (2015) (PubMed).

Lee, Chen, Huang, Stargell: "The head module of Mediator directs activation of preloaded RNAPII in vivo." dans: Nucleic acids research, Vol. 41, Issue 22, pp. 10124-34, (2013) (PubMed).

Détails sur Rpb1 CTD

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Autre désignation: RNA Pol II CTD

Sujet: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

Poids moléculaire: 210 kDa

ID gène: 5430

NCBI Accession: NP_000928