CHD1 anticorps (AA 251-467)

N° du produit ABIN2668525

Il existe 1 publication pour ce produit. L’anticorps anti-CHD1 Polyclonal Lapin est utilisé pour la détection de CHD1 dans des échantillons de Humain et Souris. Il a été validé pour WB.

Aperçu rapide pour CHD1 anticorps (AA 251-467) (ABIN2668525)

Antigène: CHD1 (Chromodomain Helicase DNA Binding Protein 1 (CHD1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHD1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-CHD1 anticorps

Épitope: AA 251-467

Fonction: CHD1 antibody (pAb)

Purification: Unpurified

Immunogène: This CHD1 antibody was raised against a recombinant protein corresponding to amino acids 251-467 of human CHD1.

Information d'application

Indications d'application: WB: 1:500 - 1:1,000 dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-CHD1 (ABIN2668525)

Skene, Hernandez, Groudine, Henikoff: "The nucleosomal barrier to promoter escape by RNA polymerase II is overcome by the chromatin remodeler Chd1." dans: eLife, Vol. 3, pp. e02042, (2014) (PubMed).

Détails sur CHD1

Antigène: CHD1 (Chromodomain Helicase DNA Binding Protein 1 (CHD1))

Autre désignation: CHD1

Sujet: CHD (Chromo-ATPase/Helicase-DNA-binding) proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation, are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD1 has been shown to interact with histone H3 trimethylated at lysine 4 (H3K4Me3) and also is required for the maintenance of stem cell pluripotency.

Poids moléculaire: 220 kDa

ID gène: 1105

NCBI Accession: NP_001261

Pathways: Chromatin Binding