POLR2A/RPB1 anticorps

N° du produit ABIN2668535

Cet anticorps anti-POLR2A/RPB1 Monoclonal Rat (Clone 1F4B6) (ABIN2668535) détecte spécifiquement POLR2A/RPB1 dans WB, IF, ICC, ChIP et ChIP-seq. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour POLR2A/RPB1 anticorps (ABIN2668535)

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp POLR2A/RPB1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Clone: 1F4B6

Détail du produit anti-POLR2A/RPB1 anticorps

Fonction: RNA pol II antibody (mAb) (Clone 1F4B6)

Purification: Protein G Chromatography

Immunogène: This antibody was raised against a synthetic peptide containing the CTD of human RNA Polymerase II.

Isotype: IgG2a

Information d'application

Indications d'application: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each ICC/IF: 5 μg/mL dilution WB*: 0.5 - 2 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. The addition of 0.1 % Tween 20 in the blocking buffer and primary antibody incubation buffer is also recommended to aid in detection by Western blot. For RNA Pol II, we also offer AbFlex RNA Pol II Recombinant Antibody (rAb). For details, see Catalog No. 91151.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur POLR2A/RPB1

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Autre désignation: POLR2A / RNA Polymerase II

Sujet: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Poids moléculaire: 240 kDa

ID gène: 5430

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways