POLR2F anticorps (C-Term)

N° du produit ABIN2668536

Cet anticorps Rat Monoclonal détecte spécifiquement POLR2F dans WB, IF, ChIP et IP. Il présente une réactivité avec des échantillons de Humain et Souris. Il a été cité dans 3+ publications.

Aperçu rapide pour POLR2F anticorps (C-Term) (ABIN2668536)

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp POLR2F est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP)

Clone: 4F8

Détail du produit anti-POLR2F anticorps

Épitope: C-Term

Fonction: RNA pol II antibody (mAb) (Clone 4F8)

Purification: Protein G Chromatography

Immunogène: This RNA pol II antibody was raised against a synthetic peptide corresponding to human RNA pol II C-terminal heptad repeat domain.

Isotype: IgG1

Information d'application

Indications d'application: For RNA Pol II, we also offer an AbFlex RNA Pol II Recombinant Antibody (rAb). For details, see Catalog No. 91151.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-POLR2F (ABIN2668536)

Catania, Pidoux, Allshire: "Sequence features and transcriptional stalling within centromere DNA promote establishment of CENP-A chromatin." dans: PLoS genetics, Vol. 11, Issue 3, pp. e1004986, (2015) (PubMed).

Dahiya, Liu, Nonnemacher, Dampier, Wigdahl: "CCAAT enhancer binding protein and nuclear factor of activated T cells regulate HIV-1 LTR via a novel conserved downstream site in cells of the monocyte-macrophage lineage." dans: PLoS ONE, Vol. 9, Issue 2, pp. e88116, (2014) (PubMed).

Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." dans: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).

Détails sur POLR2F

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Autre désignation: RNA Pol II

Sujet: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Poids moléculaire: 240 kDa

ID gène: 5430

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways