MBD3 anticorps

N° du produit ABIN2668686

Cet anticorps Souris Monoclonal détecte spécifiquement MBD3 dans WB. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour MBD3 anticorps (ABIN2668686)

Antigène: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MBD3 est non-conjugé

Application: Western Blotting (WB)

Clone: 106B691

Détail du produit anti-MBD3 anticorps

Fonction: MBD3 antibody (mAb) (Clone 106B691)

Purification: Affinity Purified

Immunogène: This MBD3 antibody was raised against a synthetic peptide corresponding to human MBD3.

Isotype: IgG

Information d'application

Indications d'application: WB: 1 - 2 μg/mL dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 μg/μL

Buffer: PBS containing 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: 4 °C,-20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur MBD3

Antigène: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Autre désignation: MBD3

Sujet: MBD3 (methyl-CpG binding domain protein 3) is a subunit of the NuRD, a multi-subunit complex containing nucleosome remodeling and histone deacetylase activities. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD3 regulates transcription by forming a Mi-2/NuRD complex with nucleosome remodeling and histone deacetylase (HDAC) activities in mammalian cells. MBD3 has been shown to interact not with 5-methylcytidine methylated DNA but with DNA containing 5-hydroxymethylcytosine (5-hmC) methylation. MDB3 co-localizes with TET1 and is required for normal levels of 5-hmC in embryonic stem cells.

Poids moléculaire: 50 kDa

ID gène: 53615

NCBI Accession: NP_001268382

Pathways: Chromatin Binding