SRF anticorps

N° du produit ABIN2668813

Il existe 2+ publications pour ce produit. L’anticorps anti-SRF Monoclonal Rat est utilisé pour la détection de SRF dans des échantillons de Humain et Souris. Il a été validé pour WB, IHC, IF, ChIP et ChIP-seq.

Aperçu rapide pour SRF anticorps (ABIN2668813)

Antigène: SRF (Serum Response Factor (SRF))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp SRF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Clone: 2C5

Détail du produit anti-SRF anticorps

Fonction: SRF antibody (mAb) (Clone 2C5)

Purification: Protein G Chromatography

Immunogène: This antibody was raised against a full-length protein corresponding to mouse SRF.

Isotype: IgG2a

Information d'application

Indications d'application: ChIP: 5 - 10 µL per ChIP ChIP-Seq: 5 - 10 µL each ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-SRF (ABIN2668813)

Werfel, Jungmann, Lehmann, Ksienzyk, Bekeredjian, Kaya, Leuchs, Nordheim, Backs, Engelhardt, Katus, Müller: "Rapid and highly efficient inducible cardiac gene knockout in adult mice using AAV-mediated expression of Cre recombinase." dans: Cardiovascular research, Vol. 104, Issue 1, pp. 15-23, (2014) (PubMed).

Koegel, von Tobel, Schäfer, Alberti, Kremmer, Mauch, Hohl, Wang, Beer, Bloch, Nordheim, Werner: "Loss of serum response factor in keratinocytes results in hyperproliferative skin disease in mice." dans: The Journal of clinical investigation, Vol. 119, Issue 4, pp. 899-910, (2009) (PubMed).

Détails sur SRF

Antigène: SRF (Serum Response Factor (SRF))

Autre désignation: SRF

Sujet: SRF (serum response factor) is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).

Poids moléculaire: 67 kDa

ID gène: 20807

NCBI Accession: NP_065239