MECP2 anticorps

N° du produit ABIN2668837

Il existe 1 publication pour ce produit. L’anticorps anti-MECP2 Monoclonal Rat est utilisé pour la détection de MECP2 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, ICC, IP, IF et ChIP.

Aperçu rapide pour MECP2 anticorps (ABIN2668837)

Antigène: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivité: Humain, Souris, Rat

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp MECP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Clone: 4H7

Détail du produit anti-MECP2 anticorps

Fonction: MeCP2 antibody (mAb) (Clone 4H7)

Purification: Protein G Chromatography

Immunogène: This MeCP2 antibody was raised against a full-length protein corresponding to rat MeCP2.

Isotype: IgG2a

Information d'application

Indications d'application: The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications: ChIP IP ICC/IF IHC WB View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-MECP2 (ABIN2668837)

Jost, Rottach, Milden, Bertulat, Becker, Wolf, Sandoval, Petazzi, Huertas, Esteller, Kremmer, Leonhardt, Cardoso: "Generation and characterization of rat and mouse monoclonal antibodies specific for MeCP2 and their use in X-inactivation studies." dans: PLoS ONE, Vol. 6, Issue 11, pp. e26499, (2011) (PubMed).

Détails sur MECP2

Antigène: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Autre désignation: MeCP2

Sujet: Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MeCP2 (methyl CpG binding protein 2, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A.

Poids moléculaire: 72 kDa

ID gène: 29386

NCBI Accession: NP_073164

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane