Histone H4 anticorps (H4K20me3)

N° du produit ABIN2668855

Il existe 3+ publications pour ce produit. L’anticorps anti-Histone H4 Monoclonal Souris est utilisé pour la détection de Histone H4 dans des échantillons de Humain et Souris. Il a été validé pour WB, ICC, ChIP et DB.

Aperçu rapide pour Histone H4 anticorps (H4K20me3) (ABIN2668855)

Antigène: Histone H4 (HISTH4)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Clone: 6F8-D9

Détail du produit anti-Histone H4 anticorps

Épitope: H4K20me3

Fonction: Histone H4K20me3 antibody (mAb) (Clone 6F8-D9)

Purification: Protein G Chromatography

Immunogène: This Histone H4 trimethyl Lys20 antibody (mAb) was raised against a peptide containing trimethyl Lys20 of Histone H4.

Isotype: IgG

Information d'application

Indications d'application: ChIP: 5 - 10 μg per ChIP WB*: 1 - 2 μg/mL dilution DB: 0.2 μg/mL dilution Slight reactivity towards histone H4 dimethyl Lys20 might be observed under certain conditions. Individual optimization may be required. *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer can be used to aid in detection by Western blot. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 0.035 % sodium azide and 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone H4 (ABIN2668855)

Gonzalez-Vasconcellos, Schneider, Anastasov, Alonso-Rodriguez, Sanli-Bonazzi, Fernández, Atkinson: "The Rb1 tumour suppressor gene modifies telomeric chromatin architecture by regulating TERRA expression." dans: Scientific reports, Vol. 7, pp. 42056, (2017) (PubMed).

Dal-Pra, Hodgkinson, Mirotsou, Kirste, Dzau: "Demethylation of H3K27 Is Essential for the Induction of Direct Cardiac Reprogramming by miR Combo." dans: Circulation research, Vol. 120, Issue 9, pp. 1403-1413, (2017) (PubMed).

Nelson, Jaber-Hijazi, Cole, Robertson, Pawlikowski, Norris, Criscione, Pchelintsev, Piscitello, Stong, Rai, McBryan, Otte, Nixon, Clark, Riethman, Wu, Schotta, Garcia, Neretti, Baird, Berger, Adams: "Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability." dans: Genome biology, Vol. 17, Issue 1, pp. 158, (2016) (PubMed).

Détails sur Histone H4

Antigène: Histone H4 (HISTH4)

Autre désignation: Histone H4

Sujet: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.

Poids moléculaire: 8 kDa

ID gène: 121504

NCBI Accession: NP_778224