Histone H4 anticorps

N° du produit ABIN2668856

Cité dans 2 publications. Cet anticorps anti-Histone H4 Monoclonal Souris (Clone MABI 0400) (ABIN2668856) détecte spécifiquement Histone H4 dans WB et ChIP. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Histone H4 anticorps (ABIN2668856)

Antigène: Histone H4 (HISTH4)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Clone: MABI 0400

Détail du produit anti-Histone H4 anticorps

Fonction: Histone H4 antibody (mAb) (Clone MABI 0400)

Purification: Protein G Chromatography

Immunogène: This antibody was raised against a synthetic peptide containing human Histone H4.

Isotype: IgG2b

Information d'application

Indications d'application: ChIP: 2 - 5 μg per ChIP WB*: 0.5 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For Histone H4, we also offer AbFlex Histone H4 Recombinant Antibody (rAb). For details, see Catalog No. 91295.

Restrictions: For Research Use only

Stockage

Concentration: 0.6 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol. 0.21M NaCl, and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone H4 (ABIN2668856)

Bulusu, Tumanov, Michalopoulou, van den Broek, MacKay, Nixon, Dhayade, Schug, Vande Voorde, Blyth, Gottlieb, Vazquez, Kamphorst: "Acetate Recapturing by Nuclear Acetyl-CoA Synthetase 2 Prevents Loss of Histone Acetylation during Oxygen and Serum Limitation." dans: Cell reports, Vol. 18, Issue 3, pp. 647-658, (2017) (PubMed).

Powers, Parvanov, Baker, Walker, Petkov, Paigen: "The Meiotic Recombination Activator PRDM9 Trimethylates Both H3K36 and H3K4 at Recombination Hotspots In Vivo." dans: PLoS genetics, Vol. 12, Issue 6, pp. e1006146, (2016) (PubMed).

Détails sur Histone H4

Antigène: Histone H4 (HISTH4)

Autre désignation: Histone H4

Sujet: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.

Poids moléculaire: 8 kDa

ID gène: 121504

NCBI Accession: NP_778224