Histone H4 anticorps (H4K12ac)

N° du produit ABIN2668859

Cet anticorps anti-Histone H4 Monoclonal Souris (Clone MABI 0412) (ABIN2668859) détecte spécifiquement Histone H4 dans WB, ChIP et DB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Histone H4 anticorps (H4K12ac) (ABIN2668859)

Antigène: Histone H4 (HISTH4)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Clone: MABI 0412

Détail du produit anti-Histone H4 anticorps

Épitope: H4K12ac

Fonction: Histone H4K12ac antibody (mAb) (Clone MABI 0412)

Purification: Protein G Chromatography

Immunogène: This antibody was raised against a synthetic peptide containing acetyl-lysine 12 of human Histone H4.

Isotype: IgG1

Information d'application

Indications d'application: ChIP: 2 - 5 μg per ChIP WB*: 0.5 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Stockage

Concentration: 0.72 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur Histone H4

Antigène: Histone H4 (HISTH4)

Autre désignation: Histone H4

Sujet: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The acetylation of histone H4 Lys12 is carried out by several histone acetyltransferases (HATs). Histone H4 Lys12 acetylation by TIP60 occurs in the nucleus, whereas Hat1-mediated acetylation of histone H4 Lys12 occurs in the cytoplasm, indicating the spatial regulation of histone acetylation. The acetylation of histone H4 Lys12 residue is commonly associated with transcriptional activation.

Poids moléculaire: 8 kDa

ID gène: 121504

NCBI Accession: NP_778224