Histone H4 anticorps (acetylated)

N° du produit ABIN2668867

Il existe 8+ publications pour ce produit. L’anticorps anti-Histone H4 Polyclonal Lapin est utilisé pour la détection de Histone H4 dans des échantillons de Humain et Souris. Il a été validé pour WB, IF, ICC, ChIP et DB.

Aperçu rapide pour Histone H4 anticorps (acetylated) (ABIN2668867)

Antigène: Histone H4 (HISTH4)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Détail du produit anti-Histone H4 anticorps

Épitope: acetylated

Fonction: Histone H4ac (pan-acetyl) antibody (pAb)

Purification: Unpurified

Immunogène: This Histone H4 pan-acetyl antibody was raised against a peptide containing the amino terminal region of Tetrahymena histone H2A acetylated at multiple lysines. (The immunogen has a high degree of homology to mammalian histone H4, so the antibody recognizes acetylated histone H4 in HeLa extracts).

Information d'application

Indications d'application: ChIP: 5 - 10 µL per ChIP ICC/IF: 1:500 - 1:2,000 dilution WB: 1:1,000 - 1:10,000 dilution

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone H4 (ABIN2668867)

Kungulovski, Nunna, Thomas, Zanger, Reinhardt, Jeltsch: "Targeted epigenome editing of an endogenous locus with chromatin modifiers is not stably maintained." dans: Epigenetics & chromatin, Vol. 8, pp. 12, (2015) (PubMed).

Xiao, Shi, Fan, Zhang, Wu, Lan, Minze, Fu, Ghobrial, Liu, Li: "GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation." dans: Nature communications, Vol. 6, pp. 8266, (2015) (PubMed).

Lehmann, Hoffmann, Koch, Ulrich, Schulz, Niegisch: "Histone deacetylase 8 is deregulated in urothelial cancer but not a target for efficient treatment." dans: Journal of experimental & clinical cancer research : CR, Vol. 33, pp. 59, (2014) (PubMed).

Li, Wu, Kim, Zhao, Hearn, Zhang, Meistrich, Mills: "Chd5 orchestrates chromatin remodelling during sperm development." dans: Nature communications, Vol. 5, pp. 3812, (2014) (PubMed).

Walker, LaFerla, Oddo, Brewer: "Reversible epigenetic histone modifications and Bdnf expression in neurons with aging and from a mouse model of Alzheimer's disease." dans: Age (Dordrecht, Netherlands), Vol. 35, Issue 3, pp. 519-31, (2013) (PubMed).

Larsson, Ulfhammer, Magnusson, Bergh, Lunke, El-Osta, Medcalf, Svensson, Karlsson, Jern: "Role of histone acetylation in the stimulatory effect of valproic acid on vascular endothelial tissue-type plasminogen activator expression." dans: PLoS ONE, Vol. 7, Issue 2, pp. e31573, (2012) (PubMed).

Fazzio, Panning: "Condensin complexes regulate mitotic progression and interphase chromatin structure in embryonic stem cells." dans: The Journal of cell biology, Vol. 188, Issue 4, pp. 491-503, (2010) (PubMed).

Gray, Pett, Ward, Winder, Stanley, Roberts, Scarpini, Coleman: "In vitro progression of human papillomavirus 16 episome-associated cervical neoplasia displays fundamental similarities to integrant-associated carcinogenesis." dans: Cancer research, Vol. 70, Issue 10, pp. 4081-91, (2010) (PubMed).

Détails sur Histone H4

Antigène: Histone H4 (HISTH4)

Autre désignation: Histone H4

Sujet: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation.

Poids moléculaire: 8 kDa

ID gène: 121504

NCBI Accession: NP_778224