Histone H4 anticorps

N° du produit ABIN2668873

Il existe 2+ publications pour ce produit. L’anticorps anti-Histone H4 Polyclonal Lapin est utilisé pour la détection de Histone H4 dans des échantillons de Humain. Il a été validé pour WB, ChIP et ChIP-seq.

Aperçu rapide pour Histone H4 anticorps (ABIN2668873)

Antigène: Histone H4 (HISTH4)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Détail du produit anti-Histone H4 anticorps

Fonction: Histone H4 antibody (pAb)

Purification: Affinity Purified

Immunogène: This Histone H4 antibody was raised against a peptide corresponding to amino acids 43- 54 of human histone H4.

Isotype: IgG

Information d'application

Indications d'application: ChIP: 2 - 10 µL per ChIP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. For Histone H4, we also offer AbFlex Histone H4 Recombinant Antibody (rAb). For details, see Catalog No. 91295.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone H4 (ABIN2668873)

Wagner, Ciszewski, Kania, Dastych: "Lactate Stimulates IL-4 and IL-13 Production in Activated HuT-78 T Lymphocytes Through a Process That Involves Monocarboxylate Transporters and Protein Hyperacetylation." dans: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 36, Issue 5, pp. 317-27, (2016) (PubMed).

Wagner, Ciszewski, Kania: "L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation." dans: Cell communication and signaling : CCS, Vol. 13, pp. 36, (2015) (PubMed).

Détails sur Histone H4

Antigène: Histone H4 (HISTH4)

Autre désignation: Histone H4

Sujet: Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression.

Poids moléculaire: 8 kDa

ID gène: 8363

NCBI Accession: NP_778224