SUZ12 anticorps

N° du produit ABIN2668948

Il existe 2+ publications pour ce produit. L’anticorps anti-SUZ12 Monoclonal Souris est utilisé pour la détection de SUZ12 dans des échantillons de Humain. Il a été validé pour IHC, IP et ChIP.

Aperçu rapide pour SUZ12 anticorps (ABIN2668948)

Antigène: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SUZ12 est non-conjugé

Application: Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 2AO9

Détail du produit anti-SUZ12 anticorps

Fonction: Suz12 antibody (mAb) (Clone 2AO9)

Purification: Protein G Chromatography

Immunogène: This Suz12 antibody was raised against a recombinant protein corresponding to full-length human Suz12.

Isotype: IgG2b

Information d'application

Indications d'application: ChIP: 2.5 - 10 μg per ChIP This antibody has been published, but has not been validated by Active Motif, for Western blot. For an antibody validated for use in Western blotting, see Catalog No. 39357. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91163.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-SUZ12 (ABIN2668948)

Pasini, Hansen, Christensen, Agger, Cloos, Helin: "Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2." dans: Genes & development, Vol. 22, Issue 10, pp. 1345-55, (2008) (PubMed).

Herranz, Pasini, Díaz, Francí, Gutierrez, Dave, Escrivà, Hernandez-Muñoz, Di Croce, Helin, García de Herreros, Peiró: "Polycomb complex 2 is required for E-cadherin repression by the Snail1 transcription factor." dans: Molecular and cellular biology, Vol. 28, Issue 15, pp. 4772-81, (2008) (PubMed).

Détails sur SUZ12

Antigène: SUZ12 (SUZ12 Polycomb Repressive Complex 2 Subunit (SUZ12))

Autre désignation: Suz12

Sujet: Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3K27me2 and Histone H3K27me3), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.

Poids moléculaire: 95 kDa

ID gène: 23512

NCBI Accession: NP_056170