EZH2 anticorps (AA 353-451)

N° du produit ABIN7938124

Cité dans 8 publications. Cet anticorps anti-EZH2 Monoclonal Souris (Clone AC22) (ABIN7938124) détecte spécifiquement EZH2 dans ChIP. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour EZH2 anticorps (AA 353-451) (ABIN7938124)

Antigène: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EZH2 est non-conjugé

Application: Chromatin Immunoprecipitation (ChIP)

Clone: AC22

Détail du produit anti-EZH2 anticorps

Épitope: AA 353-451

Fonction: EZH2 antibody (mAb) (Clone AC22)

Purification: Protein A Chromatography

Immunogène: This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2.

Isotype: IgG2a

Information d'application

Indications d'application: ChIP: 1 - 3 μg per ChIP CUT&RUN: 1 µL per 50 µL reaction This antibody has been published, but not validated by Active Motif, for Western blot. For an antibody we have tested in use in Western blotting, see Catalog No. 39933. For EZH2, we also offer an AbFlex EZH2 Recombinant Antibody (rAb). For details, see Catalog No. 91159.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-EZH2 (ABIN7938124)

Toth, Papp, Brulois, Choi, Gao, Jung: "LANA-Mediated Recruitment of Host Polycomb Repressive Complexes onto the KSHV Genome during De Novo Infection." dans: PLoS pathogens, Vol. 12, Issue 9, pp. e1005878, (2016) (PubMed).

Ranzani, Rossetti, Panzeri, Arrigoni, Bonnal, Curti, Gruarin, Provasi, Sugliano, Marconi, De Francesco, Geginat, Bodega, Abrignani, Pagani: "The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4." dans: Nature immunology, Vol. 16, Issue 3, pp. 318-325, (2015) (PubMed).

Bengani, Mendiratta, Maini, Vasanthi, Sultana, Ghasemi, Ahluwalia, Ramachandran, Mishra, Brahmachari: "Identification and Validation of a Putative Polycomb Responsive Element in the Human Genome." dans: PLoS ONE, Vol. 8, Issue 6, pp. e67217, (2013) (PubMed).

Wang, Pan, Lee, Martinez, Feng, Dong: "Cutting edge: Smad2 and Smad4 regulate TGF-β-mediated Il9 gene expression via EZH2 displacement." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 10, pp. 4908-12, (2013) (PubMed).

Rossetto, Tarrant-Elorza, Pari: "Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells." dans: PLoS pathogens, Vol. 9, Issue 5, pp. e1003366, (2013) (PubMed).

Lin, Shen, Li, Tang, Gu, Chen, Hu, Rice, Lu, Wu: "Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program." dans: The Journal of biological chemistry, Vol. 288, Issue 9, pp. 6238-47, (2013) (PubMed).

Woellmer, Arteaga-Salas, Hammerschmidt: "BZLF1 governs CpG-methylated chromatin of Epstein-Barr Virus reversing epigenetic repression." dans: PLoS pathogens, Vol. 8, Issue 9, pp. e1002902, (2012) (PubMed).

Murata, Kondo, Sugimoto, Kawashima, Saito, Isomura, Kanda, Tsurumi: "Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells." dans: Journal of virology, Vol. 86, Issue 9, pp. 4752-61, (2012) (PubMed).

Détails sur EZH2

Antigène: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Autre désignation: EZH2

Sujet: EZH2 - (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.

Poids moléculaire: 96 kDa

ID gène: 2146

NCBI Accession: NP_004447

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation