SP1 anticorps (pSer101)

N° du produit ABIN2668960

L’anticorps anti-SP1 Polyclonal Lapin est utilisé pour la détection de SP1 dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour SP1 anticorps (pSer101) (ABIN2668960)

Antigène: SP1 (Sp1 Transcription Factor (SP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SP1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-SP1 anticorps

Épitope: pSer101

Fonction: Sp1 phospho Ser101 antibody (pAb)

Purification: Affinity Purified

Immunogène: This Sp1 phospho Ser101 antibody was raised against a peptide containing phospho-serine 101 of human Sp1.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500 dilution

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur SP1

Antigène: SP1 (Sp1 Transcription Factor (SP1))

Autre désignation: Sp1

Sujet: Sp1 (specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain. Sp1 contains two SQ/TQ domains, which are found in proteins phosphorylated by the DNA-damage response transducer/effector kinase ATM. Phosphorylation of serine 101 (dependent upon ATM and ATR) is stimulated by double strand DNA breakage (DSBs) as well as by blocks to DNA replication induced by treatment with hydroxyurea or UV irradiation. Sp1 phospho Ser101 co-localizes with ATM phospho Ser1981 at DSBs, suggesting that this phosphorylated version of Sp1 plays a role in double strand break repair.

Poids moléculaire: 96 kDa

ID gène: 6667

NCBI Accession: NP_612482

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction