5-Carboxylcytosine anticorps

N° du produit ABIN2668976

Cité dans 5 publications. Cet anticorps anti-5-Carboxylcytosine Polyclonal Lapin (ABIN2668976) détecte spécifiquement 5-Carboxylcytosine dans DB, IF, ICC, IHC et WB.

Aperçu rapide pour 5-Carboxylcytosine anticorps (ABIN2668976)

Antigène: 5-Carboxylcytosine

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Dot Blot (DB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)

Détail du produit

Fonction: 5-Carboxylcytosine (5-caC) antibody (pAb)

Purification: Unpurified

Immunogène: This 5-Carboxylcytosine antibody was raised against 5-carboxylcytidine conjugated to KLH and recognizes 5-carboxylcytosine.

Information d'application

Indications d'application: DB: 1:5,000 dilution

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Référence

Eleftheriou, Pascual, Wheldon, Perry, Abakir, Arora, Johnson, Auer, Ellis, Madhusudan, Ruzov: "5-Carboxylcytosine levels are elevated in human breast cancers and gliomas." dans: Clinical epigenetics, Vol. 7, pp. 88, (2015) (PubMed).

Nettersheim, Heukamp, Fronhoffs, Grewe, Haas, Waha, Honecker, Waha, Kristiansen, Schorle: "Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development." dans: PLoS ONE, Vol. 8, Issue 12, pp. e82881, (2014) (PubMed).

Hashimoto, Pais, Zhang, Saleh, Fu, Dai, Corrêa, Zheng, Cheng: "Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA." dans: Nature, Vol. 506, Issue 7488, pp. 391-5, (2014) (PubMed).

Zhang, Szulwach, Hon, Song, Park, Yu, Lu, Dai, Wang, Street, Tan, Min, Ren, Jin, He: "Tet-mediated covalent labelling of 5-methylcytosine for its genome-wide detection and sequencing." dans: Nature communications, Vol. 4, pp. 1517, (2013) (PubMed).

Inoue, Shen, Dai, He, Zhang: "Generation and replication-dependent dilution of 5fC and 5caC during mouse preimplantation development." dans: Cell research, Vol. 21, Issue 12, pp. 1670-6, (2011) (PubMed).

Détail du antigène

Antigène: 5-Carboxylcytosine

Classe de substances: Chemical

Sujet: DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.

ID gène: 57279